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Transcription in bacteria презентация
Содержание
- 1. Transcription in bacteria
- 2. Transcription RNA synthesis
- 3. Transcription RNA bases
- 4. Transcription RNA polymerase α2ββ’ωσ Core α
- 5. Transcription Proteins that associate with RNAP during
- 6. Transcription Initiation of transcription
- 7. Transcription Promoters and σ factors
- 8. Transcription Structure of initiation complex Mg2+ -35 -10 σ2 TG σ4 GGGA σ1 Core Core
- 9. Transcription Structure of initiation complex Mg2+ -10 σ2 TG GGGA σ1 Core Core RNA
- 10. Transcription Elongation Elongation Pausing, regulation or secondary
- 11. Transcription GreA use the tunnel to access RNAP active center
- 12. Transcription Termination ρ-dependent ρ-independent
- 13. Transcription ρ-factor
- 14. Regulation of transcription Regulation by σ-factor change
- 15. Regulation of transcription DNA looping
- 16. Regulation of transcription Regulation of stationary σ-factor expression
- 17. Regulation of transcription Regulation by DNA binding
- 18. Regulation of transcription Regulation by DNA binding
- 19. Regulation of transcription Regulation by DNA binding
- 20. Regulation of transcription Regulation by DNA binding
- 21. Regulation of transcription Regulation by DNA binding
- 22. Regulation of transcription Regulation by DNA binding regulator proteins Transcription factor activity change by localization
- 23. Regulation of transcription Regulation by low molecular weight compounds Low UTP Excess UTP: abortive transcription
- 24. Regulation of transcription Regulation by low molecular
- 25. Regulation of transcription Regulation of elongation and
- 26. Regulation of transcription Regulation of elongation and
- 27. Regulation of transcription Regulation of elongation and
- 28. Regulation of transcription Regulation of elongation and
- 29. Regulation of transcription Regulation of elongation and termination
- 30. Regulation of transcription Regulation of elongation and termination Antitermination Q Antitermination N
- 31. Regulation of transcription Regulation of initiation
- 32. Regulation of transcription Regulation of elongation and
- 33. Examples of the transcriptional control Bacterial flagella biosynthesis Flagella is expensive and energy consuming machinery
- 34. Examples of the transcriptional control Bacterial flagella biosynthesis Many regulators control flagella biogenesis
- 35. Examples of the transcriptional control Bacterial flagella
- 36. Examples of the transcriptional control Bacterial flagella
- 37. Examples of the transcriptional control Bacterial flagella
- 38. Examples of the transcriptional control Transcriptional control of phage λ Genome
- 39. Examples of the transcriptional control Transcriptional control
- 40. Examples of the transcriptional control Transcriptional control
- 41. Examples of the transcriptional control Transcriptional control
- 42. Examples of the transcriptional control Transcriptional control
- 43. SOS response Activated RecA cleaves LexA repressor And so what?
- 44. Examples of the transcriptional control Transcriptional control of phage λ Activated RecA cleaves CI
- 45. Examples of the transcriptional control Transcriptional control
- 46. Examples of the transcriptional control Transcriptional control of phage λ Cro re-starts lytic pathway
- 47. Examples of the transcriptional control Transcriptional control
- 48. Examples of the transcriptional control Transcriptional control
- 49. Examples of the transcriptional control Transcriptional control
- 50. Examples of the transcriptional control Transcriptional control
- 51. Examples of the transcriptional control Transcriptional control
- 52. Examples of the transcriptional control Transcriptional control
Transcription RNA synthesis
Слайд 4Transcription
RNA polymerase
α2ββ’ωσ
Core
α 40 kD
β 155 kD
β’ 165 kD
ω
10 kD
RNA polymerase
σ 70 major
σ 20 Fe2+ transport
σ 28 flagella
σ 32 heat shock
σ 54 nitrogen limitation
σ 24 periplasm stress
σ 38 stationary phase
Слайд 5Transcription
Proteins that associate with RNAP during elongation
NusA 55kD pausing,
antitermination
NusG 21kD suppression of pausing, antitermination
GreA 18kD cleavage of 2-3 n. in stalled RNAP elongation complexes
GreB 19kD cleavage of 9 n. in stalled RNAP elongation complexes
Mfd 130kD release of RNAP blocked by DNA lesions, activation of UvrABC
NusG 21kD suppression of pausing, antitermination
GreA 18kD cleavage of 2-3 n. in stalled RNAP elongation complexes
GreB 19kD cleavage of 9 n. in stalled RNAP elongation complexes
Mfd 130kD release of RNAP blocked by DNA lesions, activation of UvrABC
Termination
factor
ρ 47 kD, hexamer, ATPase
Слайд 10Transcription
Elongation
Elongation
Pausing, regulation
or secondary structure formation
Release of stalled complexes
Release of RNAP stalled
at DNA lesions
Слайд 17Regulation of transcription
Regulation by DNA binding regulator proteins
lac operon:
Glucose is preferable
energy source
Lactose could be utilized if available
Lactose could be utilized if available
Jacob, Monod
Nobel Prize
Слайд 18Regulation of transcription
Regulation by DNA binding regulator proteins
Glucose
No lactose
No glucose
No lactose
Glucose
Lactose
No
glucose
Lactose
Слайд 19Regulation of transcription
Regulation by DNA binding regulator proteins
How activation works?
Activation via
α-subunit CTD
Слайд 20Regulation of transcription
Regulation by DNA binding regulator proteins
How activation works?
Activation/repression via
DNA structure change
Слайд 21Regulation of transcription
Regulation by DNA binding regulator proteins
Complex regulation
No oxygen
Nir-operon
No oxygen
Nitrite
ions present
Слайд 22Regulation of transcription
Regulation by DNA binding regulator proteins
Transcription factor activity change
by localization
Слайд 23Regulation of transcription
Regulation by low molecular weight compounds
Low UTP
Excess UTP: abortive
transcription
Слайд 25Regulation of transcription
Regulation of elongation and termination
Attenuation of E. coli Trp
operon
Low Trp concentration
High Trp concentration
Слайд 26Regulation of transcription
Regulation of elongation and termination
Attenuation of E. coli pyrimidine
operon
Low UTP concentration
High UTP concentration
Слайд 27Regulation of transcription
Regulation of elongation and termination
Attenuation of B. subtilis Trp
operon
Low Trp concentration
High Trp concentration
Слайд 28Regulation of transcription
Regulation of elongation and termination
Attenuation of B. subtilis TyrRS
gene
Low aminoacylation
High aminoacylation
Слайд 30Regulation of transcription
Regulation of elongation and termination
Antitermination Q
Antitermination N
Слайд 32Regulation of transcription
Regulation of elongation and termination
Attenuation
- translation
speed
-transcription speed
-protein binding
-RNA binduing
-small molecule binding
Antitermination
- RNA binding protein antiterminator
- DNA binding protein antiterminator
Слайд 33Examples of the transcriptional control
Bacterial flagella biosynthesis
Flagella is expensive and energy
consuming machinery
Слайд 34Examples of the transcriptional control
Bacterial flagella biosynthesis
Many regulators control flagella biogenesis
Слайд 35Examples of the transcriptional control
Bacterial flagella biosynthesis
FlhDC activates a set of
early promoters
Слайд 36Examples of the transcriptional control
Bacterial flagella biosynthesis
Positive feedback loop starts after
hook/basal body assembly
Слайд 37Examples of the transcriptional control
Bacterial flagella biosynthesis
Flagellin biosynthesis is activated after
assembly of a functional hook and basal body
Слайд 39Examples of the transcriptional control
Transcriptional control of phage λ
Early genes
Making a
choice
Hunger
Lysogeny
Слайд 40Examples of the transcriptional control
Transcriptional control of phage λ
CII activates genes
required for lysogeny
Слайд 41Examples of the transcriptional control
Transcriptional control of phage λ
CI inactivate everything
but its own gene
Слайд 42Examples of the transcriptional control
Transcriptional control of phage λ
CI protein: positive
and negative feedback loops
Слайд 44Examples of the transcriptional control
Transcriptional control of phage λ
Activated RecA cleaves
CI
Слайд 45Examples of the transcriptional control
Transcriptional control of phage λ
Cro inactivate CI
expression
(positive feedback loop = all-or-non decision)
Слайд 46Examples of the transcriptional control
Transcriptional control of phage λ
Cro re-starts lytic
pathway
Слайд 47Examples of the transcriptional control
Transcriptional control of phage λ
Expression of genes
necessary for replication
… and Q
Слайд 48Examples of the transcriptional control
Transcriptional control of phage λ
Q inactivate tt
terminator
… and allows expression of genes necessary for virion packing and cell lysis
Слайд 49Examples of the transcriptional control
Transcriptional control of phage T4
ADP-ribosilation of α-CTD
abolish interaction with UP elements and protein activators
Слайд 50Examples of the transcriptional control
Transcriptional control of phage T4
Further ADP-ribosylation of
α-CTD
- AsiA inhibit -35 box recognition
-MotA interacts with middle gene promoters
-Alc blocks transcription on DNA without 5hmC
Слайд 51Examples of the transcriptional control
Transcriptional control of phage T4
Late genes use
gp55/gp33 sigma and sliding clamp as enhancer
Слайд 52Examples of the transcriptional control
Transcriptional control of phage T4
T4 late transcription
activation via sliding clamp
