SNAP i.d. Workflow Value Proposition презентация

Value Proposition

Reduces incubation times in Western Blotting
Compatible with all reagents and membranes
No directly competitive product on the market

Western Blotting Protocol

Higher quality blots with Immunodetection occurring in 30 min vs. 4 hrs

SNAP i.d.™

less than 30 min

5%NFDM

0.05% NFDM

Standard

Blot Holder, 20/pk
Antibody Collection Tray,
20/pk

7.5 cm x 8.8 cm

4.6 cm x 8.8 cm

3.2 cm x 8.8 cm

Stopper (XX1004708)

Silicone Tubing
(XX7100004)

Vacuum source
must provide
4” Hg (135 millibar)

Distributor

3) Place Blotted Membrane

4) Place Spacer

5) Close Blot Holder

6) Turn over Blot Holder

Solution
3b) Turn on Vacuum

4) Turn off Vacuum

5a) Add Primary Antibody
5b) Incubate 10 min
5c) Turn on Vacuum

6a) Add wash solution (3x)
6b) Turn off Vacuum

Repeat steps 4-6 for 2° Ab

your blots
And do it FAST!

Results for your afternoon meeting! (Customer quote)

Superior Blots in a SNAP!

have the time to do this tedious process.
SNAP i.d. makes optimization easy by allowing 6 blots to be processed simultaneously in only 30 minutes!

What do they typically optimize?
Antibody concentrations/dilutions
Detection reagent conditions
Target protein concentrations (more rarely)
Blocking conditions (a little secret to sensitivity)

SNAP i.d. permits fast optimization for better results.
Point out the Immunodetection handbook Western blotting section for helpful hints.

reagent conditions to accomplish. It’s a passive process!
SNAP i.d. actively drives reagents through the membrane to increase the quality of the blots and increase the speed of immunodetection!
It’s a combination of reagent flows and concentrations

Vs.

Standard ‘rocking’ of reagents

Actively drive reagents with vacuum flow

into membrane

Standard

Vacuum

Reagents penetrate more of the membrane 3D structure where the proteins are blotted.
Result = Increase quality of the blot in a SNAP!

higher sensitivity
Can use 1/10th-1/100th less concentrated blocking solution to minimize overblocking
Actively driven vacuum flow coats inner surfaces of membrane in 20 sec

GAPDH

5%NFDM

0.5% NFDM

0.1% NFDM

0.05% NFDM

Standard

1 2 3 4 5 6 7 8

1 2 3 4 5 6 7 8

1 2 3 4 5 6 7 8

1 2 3 4 5 6 7 8

i.d. User Guide

of the membrane
Yields lower backgrounds ?which may yield higher Sensitivity

GAPDH

5%NFDM

0.5% NFDM

0.1% NFDM

0.05% NFDM

Standard

1 2 3 4 5 6 7 8

1 2 3 4 5 6 7 8

1 2 3 4 5 6 7 8

1 2 3 4 5 6 7 8

clogging of blot holder
Antibody concentrations are increased to speed up reaction kinetics

prevent clogging of flow distributor
To minimize overblocking and maximize sensitivity

Rule of 3.5

1° & 2° Ab concentration
and volume

Blocking concentration

Use 1/3 the volume of Ab at 3x concentration
Concentration: Drives Ab incubation kinetics faster
Dead volume: Minimizes Ab consumption

Works: Time savings

Western Blotting Protocol

20 sec

10 min

1 min

10 min

1 min

Standard

Electro-
phoresis

Membrane
Transfer

Blocking

Antibody
Addition

Detection

Sample
Prep

SNAP i.d.™

22 min

4 Hrs

Vs.