into many sites
Transposase enzyme cuts out mobile genetic elements and insert them into specific sites.
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Transpositional site-specific recombination презентация
Содержание
- 1. Transpositional site-specific recombination
- 2. Three of the many types of mobile
- 4. Cut and Paste Transposition DNA-only
- 5. The structure of the central intermediate formed by transposase (integrase)
- 6. Replicative Transposition
- 7. Retrovirus-based Transposition Retroviral-like retrotransposition
- 9. Reverse Transcriptase From RNA to DNA
- 10. Non-retroviral retrotransposition L1 Element
- 11. Conservative Site Specific Recombination Integration vs. inversion Notice the arrows of directions
- 12. Bacteriophase Lambda
- 13. Genetic Engineering to control Gene expression
- 14. Summary DNA site-specific recombination transpositional; conservative
- 16. RNA splicing reactions
- 17. 3 Important sequences for Splicing to occur R: A or G; Y: C or U
- 18. RNA Splicing mechanism BBP: branch-point binding protein
- 20. Further mechanism to mark Exon and Intron
- 21. Consensus sequence for 3’ process AAUAAA: CstF
- 23. The Genetic Code
- 24. The Reading Frames
- 25. tRNA (clover leaf shape with four strands folded, finally L-shape)
- 26. tRNA and mRNA pairing
Three of the many types of mobile genetic elements found in bacteria Transposase gene: encoding enzymes for DNA breakage and joining Red segments: DNA sequences as recognition sites for enzymes
Слайд 1Transpositional site-specific recombination
Modest target site selectivity and insert mobile genetic elements
Слайд 2Three of the many types of mobile genetic elements found in
bacteria
Transposase gene: encoding enzymes for DNA breakage and joining
Red segments: DNA sequences as recognition sites for enzymes
Yellow segments: antibiotic genes
Transposase gene: encoding enzymes for DNA breakage and joining
Red segments: DNA sequences as recognition sites for enzymes
Yellow segments: antibiotic genes
Слайд 11Conservative Site Specific Recombination
Integration vs. inversion
Notice the arrows of directions
Слайд 14Summary
DNA site-specific recombination
transpositional; conservative
Transposons: mobile genetic elements
Transpositional: DNA only transposons, retroviral-like
retrotransposons, nonretroviral retrotransposons
Слайд 18RNA Splicing mechanism
BBP: branch-point binding protein
U2AF: a helper protein
snRNA: small nuclear
RNA
snRNP: small nuclear ribonucleoprotein
Components for splicesome
snRNP: small nuclear ribonucleoprotein
Components for splicesome
Слайд 20Further mechanism to mark Exon and Intron difference
CBC: capping binding complex
hnRNP:
heterogeneous nuclear ribonucleoprotein, binding to introns
SR: rich in serine and arginines, binding to exons
SR: rich in serine and arginines, binding to exons
Слайд 21Consensus sequence for 3’ process
AAUAAA: CstF (cleavage stimulation factor F)
GU-rich sequence:
CPSF (cleavage and polyadenylation specificity factor)
