Transpositional site-specific recombination презентация

Three of the many types of mobile genetic elements found in bacteria Transposase gene: encoding enzymes for DNA breakage and joining Red segments: DNA sequences as recognition sites for enzymes

Слайд 1Transpositional site-specific recombination
Modest target site selectivity and insert mobile genetic elements

into many sites
Transposase enzyme cuts out mobile genetic elements and insert them into specific sites.


Слайд 2Three of the many types of mobile genetic elements found in

bacteria

Transposase gene: encoding enzymes for DNA breakage and joining
Red segments: DNA sequences as recognition sites for enzymes
Yellow segments: antibiotic genes

Слайд 4Cut and Paste Transposition
DNA-only


Слайд 5The structure of the central intermediate formed by transposase (integrase)


Слайд 6Replicative Transposition


Слайд 7Retrovirus-based Transposition
Retroviral-like retrotransposition


Слайд 9Reverse Transcriptase
From RNA to DNA


Слайд 10Non-retroviral retrotransposition
L1 Element


Слайд 11Conservative Site Specific Recombination
Integration vs. inversion
Notice the arrows of directions


Слайд 12Bacteriophase Lambda


Слайд 13Genetic Engineering to control Gene expression


Слайд 14Summary

DNA site-specific recombination
transpositional; conservative
Transposons: mobile genetic elements
Transpositional: DNA only transposons, retroviral-like

retrotransposons, nonretroviral retrotransposons

Слайд 16RNA splicing reactions


Слайд 173 Important sequences for Splicing to occur
R: A or G; Y:

C or U

Слайд 18RNA Splicing mechanism
BBP: branch-point binding protein
U2AF: a helper protein

snRNA: small nuclear

RNA
snRNP: small nuclear ribonucleoprotein
Components for splicesome


Слайд 20Further mechanism to mark Exon and Intron difference
CBC: capping binding complex
hnRNP:

heterogeneous nuclear ribonucleoprotein, binding to introns
SR: rich in serine and arginines, binding to exons

Слайд 21Consensus sequence for 3’ process
AAUAAA: CstF (cleavage stimulation factor F)
GU-rich sequence:

CPSF (cleavage and polyadenylation specificity factor)

Слайд 23The Genetic Code


Слайд 24The Reading Frames


Слайд 25tRNA (clover leaf shape with four strands folded, finally L-shape)


Слайд 26tRNA and mRNA pairing


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