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DNA and Replication презентация
Содержание
- 1. DNA and Replication
- 2. Replication Facts DNA has to be copied
- 3. Semiconservative Model of Replication Idea presented by
- 4. Remember HOW the Carbons Are Numbered!
- 5. copyright cmassengale
- 6. Remember the Strands are Antiparallel
- 7. DNA Replication Begins at Origins of Replication
- 8. DNA Replication Enzyme Helicase unwinds and separates
- 9. DNA Replication Before new DNA strands can
- 10. DNA Replication DNA polymerase can only add
- 11. Synthesis of the New DNA Strands The
- 12. Synthesis of the New DNA Strands The
- 13. Lagging Strand Segments Okazaki Fragments - series
- 14. Joining of Okazaki Fragments The enzyme Ligase
- 15. Replication of Strands copyright cmassengale Replication Fork Point of Origin
- 16. copyright cmassengale
Replication Facts DNA has to be copied before a cell divides (before mitosis) New cells will need identical DNA strands Replication of DNA is copying of DNA
Слайд 2Replication Facts
DNA has to be copied before a cell divides (before
mitosis)
New cells will need identical DNA strands
Replication of DNA is copying of DNA
New cells will need identical DNA strands
Replication of DNA is copying of DNA
Слайд 3Semiconservative Model of Replication
Idea presented by Watson & Crick
The two strands
of the parental molecule separate, and each acts as a template for a new complementary strand
New DNA consists of 1 PARENTAL (original) and 1 NEW strand of DNA
New DNA consists of 1 PARENTAL (original) and 1 NEW strand of DNA
copyright cmassengale
Слайд 7DNA Replication
Begins at Origins of Replication
Two strands open forming Replication Forks
(Y-shaped region)
New strands grow at the forks
New strands grow at the forks
Слайд 8DNA Replication
Enzyme Helicase unwinds and separates the 2 DNA strands by
breaking the weak hydrogen bonds
copyright cmassengale
Слайд 9DNA Replication
Before new DNA strands can form, there must be RNA
primers present to start the addition of new nucleotides
Primase is the enzyme that synthesizes the RNA Primer
DNA polymerase can then add the new nucleotides
Primase is the enzyme that synthesizes the RNA Primer
DNA polymerase can then add the new nucleotides
copyright cmassengale
Слайд 10DNA Replication
DNA polymerase can only add nucleotides to the 3’ end
of the DNA
This causes the NEW strand to be built in a 5’ to 3’ direction
This causes the NEW strand to be built in a 5’ to 3’ direction
copyright cmassengale
Direction of Replication
Слайд 11Synthesis of the New DNA Strands
The Leading Strand is synthesized as
a single strand from the point of origin toward the opening replication fork
Слайд 12Synthesis of the New DNA Strands
The Lagging Strand is synthesized discontinuously
against overall direction of replication
This strand is made in MANY short segments It is replicated from the replication fork toward the origin
This strand is made in MANY short segments It is replicated from the replication fork toward the origin
copyright cmassengale
Слайд 13Lagging Strand Segments
Okazaki Fragments - series of short segments on the
lagging strand
Must be joined together by an enzyme
Must be joined together by an enzyme
Слайд 14Joining of Okazaki Fragments
The enzyme Ligase joins the Okazaki fragments together
to make one strand
